Gel Electrophoresis Term paper
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Procedure
We began the experiment by sealing the ends of the gel-casting tray with tape, and inserting the comb. We placed the tray out of the way to make sure that it was not disturbed. We poured about 6mm of agarose solution into the casting tray. The gel covered only about 1/2 the height of the combs teeth. We removed large bubbles with the tip of a transfer pipet while the gel was still a liquid. While waiting about ten minutes for the gel to solidify, we made sure not to move the casting tray. After the gel solidified we unsealed the ends of the casting tray. We placed the tray in the gel box, so that the comb was at the negative end. We poured 250ml of tris-borate-EDTA (TBE) buffer into the gel box to a level that covered the entire surface of the gel. We gently removed the comb, making sure not to rip the wells. The sample...
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