To Investigate The Effect Of Temperature On The Enzyme Catalase Essay

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The Effect Of Varying Enzyme Concentration On The Breakdown Of Hydrogen Peroxide In The Presence Of
Hypothesis – ;Hydrogen peroxide will breakdown to oxygen and water in the presence of Catalase. The reaction will increase with increasing enzyme concentration when molecules of hydrogen peroxide are ...

To investigate the effect of temperature on the enzyme Catalase.

 

To investigate the effect of temperature on the enzyme Catalase.

INTRODUCTION

††††††††† The aim of this experiment is to determine the effects of varying temperatures on enzyme (Catalase). We will conclude the experiment by establishing the enzymes optimum temperature (the temperature at which it works best at). We will also carry out some simple experiments using the substrate Hydrogen Peroxide.

Hydrogen Peroxide + Catalase ------> ;Water + Oxygen

Biology - Effect Of Concentration Of Substrate On Rate Of An Enzyme-Controlled Reaction
An Investigation into how the Concentration of Substrate will affect the Rate of an Enzyme Controlled Reaction Introduction I am going to investigate how the concentration of hydrogen peroxide affects ...

2H̶̶̶̶̶̶̶̶₂O₂ ;------->H₂O + O₂ ;+ heat

PLAN

††††††††† The aim of this experiment is to determine the effect of varying temperature of enzyme Catalase.

Apparatus††††††††††††††††††††††††††††††††††††††††††††††††††††††††††† Diagram

.Water bath

.Thermometer

.Pipette

.Stop Clock

.Measuring cylinder

.Inverted cylinder

.Inverted measuring cylinder

.Conical Flask/bung

.Delivery tube

Using the equipment safely

††††††††† It is important that we use the apparatus carefully, as safety will be an issue through out the whole experiment. We will wear goggles and an apron, to protect our eyes and clothes. As we are

Enzyme Action: Testing Catalase Activity
Enzyme Action: Testing Catalase Activity Introduction: Enzymes are proteins that serve as catalysts and accelerate chemical reactions in living organisms. Enzymes accomplish this by lowering the energy of activation of ...
using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they donít come in contact with our eyes and skin. We also must be careful when using hot water, being careful not to burn ourselves. Therefore through out the whole experiment we shall be very careful in our actions around the lab.

††††††††† Catalase is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and oxygen. We will measure the amount of Oxygen

ph and Hydrogen Peroxide
I am going to investigate whether pH effects the rate at which catalase decomposes hydrogen peroxide. ‚ÄėCatalase is a biological enzyme (enzymes work best at a certain pH) found in ...
released from the Hydrogen Peroxide. In order to do this we will use an inverted measuring cylinder. This piece of apparatus measures the amount of Oxygen given off, measuring in (ml). This will help us measure the amount of Oxygen more accurately.

††††††††† To make the test fair, the following parameters must remain constant during the experiment. These parameters are water, Hydrogen Peroxide, Catalase, room temperature and the duration of the reactions. If the reaction reacts too quickly we may

The Effect Or Varying Enzyme Concentration On The Breakdown Of Hydrogen Peroxide In The Presence of
Hypothesis ‚Äď Hydrogen peroxide will breakdown to oxygen and water in the presence of Catalase.¬† The reaction will increase with increasing enzyme concentration when molecules of hydrogen peroxide are freely ...
dilute the solution. By insuring this test is fair, we will gain accurate results.

Variables

†††††††

Dependant Variable: rate of breakdown of Hydrogen Peroxide, time

Independent Variable: temperature of solution.

Control Variable: Volume of Hydrogen Peroxide, volume of Catalase solution, concentration of Hydrogen Peroxide, concentration of Catalase.

HYPOTHESIS

††††††††† I predict that the breakdown† of Hydrogen Peroxide will be quicker when the temperature is increased until it exceeds 40°C. I predict that the enzymes optimum temperature is 23°C (room temperature)

BY01 Enzyme Catalase
INTRODUCTION The enzyme catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown here, 2H2O2-------------------*2H2O+O2. It is one of the fastest known enzymes and its ...
. This is because a rise in temperature increases the rate of most chemical reactions and a fall in temperature will slow them down. In many cases a rise in 10°C will double the rate of reaction in a cell. This is particle theory. I am going to investigate the temperature at which reactions occur. I predict an increase in temperature will result in an increase in kinetic energy. Since the speed of particles increases, they should collide more often
Enzyme Catalase
INTRODUCTION The enzyme catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown here, 2H2O2-------------------*2H2O+O2. It is one of the fastest known enzymes and its turnover ...
and therefore the speed of reaction increases. The particles will also have more energy thereby speeding up the reaction even more. It has been suggested that for every 10 degree rise in temperature, the speed of the reaction will double.

††††††††† At low temperatures particles of reacting substances do not have much energy. However, when the substances are heated, the particles take in energy. This causes them to move faster and collide more often. The collisions have more energy, so

The effect of hydrogen peroxide on catalase if you change the temperature
AIM Effect of temperature of the action of the Enzyme Catalase. PLANNING Background Knowledge An enzyme is a biological catalyst, it alter the rate of reaction without being changed itself. ...
more of them are successful. Therefore the rate of reaction increases.

††††††††† The more successful the collisions are the faster the reaction.

††††††††† The same can be said for reactions controlled by enzymes, but because enzymes are proteins if the temperature exceeds 50°C the enzyme will be denatured and will no longer work. For this reason few cells can tolerate temperatures higher than approximately 45°C.

††††††††† Enzymes are specific in the reactions they catalyse, much more so than inorganic Catalysts.

Catalase and Hydrogen Peroxide SC1
Aim: To investigate the effect of temperature on the enzyme catalase. Prediction: Using my existing scientific knowledge, I predict that as I raise the temperature to 30, 35, and 40, ...
Normally, a given enzyme will Catalyse only one reaction, or type of reaction. The enzyme has an active site that helps it to recognise its substrate in a very specific way. Just like a key only fits into a specific lock, each enzyme has its own specific lock, each enzyme has its own specific substrate. This is called the lock and key theory. The enzymes never actually get consumed in the process, they just increase the rate of reactions.

†††††††††

Concentration of Substrate
I have to plan and carry out an experiment to investigate the way in which concentration of a substrate affects the rate of an enzyme-catalysed reaction. I will need ...
When enzymes denature the heat starts to destroy their shape and structure. The shape of the enzyme is so important to its working that any change in the shape of the molecules will make them less effective or stop them working completely. Therefore I predict that by heating the Hydrogen Peroxide, when it reacts with the enzyme the shape of the enzyme will be ruined due to the high temperature.

††††††††† So the higher the temperature of the Hydrogen Peroxide

Hydrogen Peroxide and Catalase
For this investigation I have been asked to investigate (by experimentation) the effect of substrate concentrations on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme ...
and Catalase solution, the less effective the reaction will be.

THE EQUATION

SUBSTRATE† ENZYME† PRODUCTS

2H₂O₂††††† Catalase†† 2H₂O + O₂ ;+ heat

 
†††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††

We will be measuring three main factors:-

.Temperature-the optimum temperature of the enzyme.

.Oxygen-the amount released from the Hydrogen peroxide.

.Time- the duration of the reaction.

††††††††† The room temperature may

An investigation into the (factors that effect) rate of reaction of the enzyme catalase
  IntroductionIn this experiment I will attempt to investigate the factors that effect the rate of reaction of the enzyme catalase. ¬†¬†¬†¬†¬† Catalyse is an enzyme found in food ...
be a factor that will affect our results. Therefore we will have to try our bests to insure the test is carried out under the same conditions. This includes the room temperature.

††††††††† To make it fair we will allow the reactions to have the same amount of time to occur. We will change the TEMPERATURE of the Hydrogen Peroxide.

This is what our results table will look likeÖ

TIME (mins)

TEMPERATURE

(°c)

AMOUNT OF O₂ ;RELEASED (ml)

Test 1

Test 2

Test 3

4

4

4

4

4†

4

0°c (crushed ice)

5°c

23°c (room temp)

40°c

50°c

100°c(boiling point)

††††††††† As you can see from the results table we will be taking six measurements of the temperature ranging from (0°c to 100 °c). I predict that the enzyme will be totally denatured at 100°c and will no longer be active. We also predict that the optimum temperature of this enzyme is 23°c (room

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